Amongst other recent films, she starred in the 1997 British BDSM/fetish comedy film Preaching to the Perverted as the New York dominatrix "Tanya Cheex".

Guinevere Turner and I Shot Andy Warhol director Mary Harron wrote the screenplay which ended up being selected for the film version of Bret Easton Ellis' American Psycho. She has a brief cameo in the film, in which she delivers the in-joke line "What makes you even think I'm a lesbian?" This line is a further in joke, referencing Sarah Lawrence College from which she graduated.

Turner emerged on the scene with the groundbreaking film Go Fish, which she co-wrote and co-produced with her then-girlfriend, Rose Troche. Turner also starred in the film, portraying a young woman whose friends help her find a new girlfriend. Director Kevin Smith was a fan of the movie, particularly a scene in it wherein, in an imagined sequence, some of a character's friends chastise her for "selling out" and sleeping with a guy, and used it as an inspiration for his rather unique take on a similar theme in his own film Chasing Amy. Turner later appeared in Smith's Dogma, in a small role as a bus clerk.

A writer and story editor for the first two seasons of The L Word, Turner also made several memorable guest appearances on the show as Alice Pieszecki's screenwriter ex-girlfriend. Recently Turner wrote the script for BloodRayne directed by Uwe Boll, and the script for "The Notorious Bettie Page", with Mary Harron, who directed it.