She is best known for playing Rosalind Shays (the tough, sharp litigator who plummeted to her death down an empty elevator shaft in a famous/infamous 1991 episode) on L.A. Law, and as Dr. Katherine Pulaski in the second season of Star Trek: The Next Generation.

She was also a guest star in the episodes "Return to Tomorrow" and "Is There in Truth No Beauty?" of the original Star Trek; only 32 actors have appeared in two generations of the franchise.

Muldaur earned two Emmy-nominations for her role on L.A. Law. The scene where Rosalind and Leland were discovered in bed was ranked as the 38th greatest moment in television in an issue of EGG magazine.

A graduate of Sweet Briar College, Muldaur was at one point a board member of the Screen Actors Guild and later the president of the Academy of Television Arts and Sciences.

Retired from acting, Diana Muldaur is a dog breeder, owner, and judge.

She is currently married to Robert Dozier; she was previously married to James Vickery until his death. She has no children.

She is the older sister of singer/songwriter/ Geoff Muldaur, and the aunt of Singer/songwriter Jenni Muldaur and singer/songwriter Clare Muldaur (Clare and the Reasons).